Logo
Login Sign Up
Current Revision

ASTM E1463-92(2012)

Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)
Best Price Guarantee
Instant

$125.00

2-5 Days

$125.00

SAVE 10%

$225.00


Sub Total (1 Item(s))

$ 0.00

Estimated Shipping

$ 0.00

Total (Pre-Tax)

$ 0.00


View in Library
or
ASTM International Logo

ASTM E1463-92(2012)

Standard Guide for Conducting Static and Flow-Through Acute Toxicity Tests With Mysids From the West Coast of the United States (Withdrawn 2021)

PUBLISH DATE 2012
PAGES 23
ASTM E1463-92(2012)

1.1 This guide describes procedures for obtaining data concerning the adverse effects of a test material (not food) added to marine and estuarine waters on certain species of marine and estuarine mysids during 96 h of continuous exposure. Juvenile mysids used in these tests are taken from cultures shortly after release from the brood and exposed to varying concentrations of a toxicant in static or flow-through conditions. These procedures will be useful for conducting toxicity tests with other species of mysids, although modifications might be necessary.

1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests with other species of mysids.

1.3 The procedures given in this guide are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications these procedures can be used to conduct acute tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, oil drilling fluids, produced water from oil well production, and other types of industrial wastes.

1.4 Results of acute toxicity tests with toxicants experimentally added to salt and estuarine waters should usually be reported in terms of a LC50 (median lethal concentration).

1.5 This guide is arranged as follows:

Referenced Documents 2
Terminology 3
Summary of Guide 4
Significance and Use 5
Apparatus 6
 Facilities 6.1
 Construction Materials 6.2
 Metering Systems 6.3
 Test Chambers 6.4
 Cleaning 6.5
 Acceptability 6.6
Safety Precautions 7
Dilution Water 8
 Requirements 8.1
 Source 8.2
 Treatment 8.3
 Characterization 8.4
Test Material 9
 General 9.1
 Stock Solution 9.2
 Test Concentrations 9.3
Test Organisms10
 Species10.1
 Age10.2
 Source10.3
 Brood Stock10.4
 Food10.5
 Handling10.6
 Harvesting Young10.7
 Quality10.8
Procedure11
 Experimental Design11.1
 Dissolved Oxygen11.2
 Temperature11.3
 Loading11.4
 Salinity11.5
 Light11.6
 Beginning of Test11.7
 Feeding11.8
 Duration of Test11.9
 Biological Data11.10
 Other Measurements11.11
Analytical Methodology12
Acceptability of Test13
Interpretation of Results14
Report15
Appendixes 
Holmesimysis costataX1
Neomysis mercedisX2

1.6 The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

1.1 This guide describes procedures for obtaining data concerning the adverse effects of a test material (not food) added to marine and estuarine waters on certain species of marine and estuarine mysids during 96 h of continuous exposure. Juvenile mysids used in these tests are taken from cultures shortly after release from the brood and exposed to varying concentrations of a toxicant in static or flow-through conditions. These procedures will be useful for conducting toxicity tests with other species of mysids, although modifications might be necessary.

1.2 Modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparisons of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting acute tests with other species of mysids.

1.3 The procedures given in this guide are applicable to most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. With appropriate modifications these procedures can be used to conduct acute tests on factors such as temperature, salinity, and dissolved oxygen. These procedures can also be used to assess the toxicity of potentially toxic discharges such as municipal wastes, oil drilling fluids, produced water from oil well production, and other types of industrial wastes.

1.4 Results of acute toxicity tests with toxicants experimentally added to salt and estuarine waters should usually be reported in terms of a LC50 (median lethal concentration).

1.5 This guide is arranged as follows:

  • Referenced Documents - Section 2
  • Terminology - Section 3
  • Summary of Guide - Section 4
  • Significance and Use - Section 5
  • Apparatus - Section 6
    • Facilities - Section 6.1
    • Construction Materials - Section 6.2
    • Metering Systems - Section 6.3
    • Test Chambers - Section 6.4
    • Cleaning - Section 6.5
    • Acceptability - Section 6.6
  • Safety Precautions - Section 7
  • Dilution Water - Section 8
    • Requirements - Section 8.1
    • Source - Section 8.2
    • Treatment - Section 8.3
    • Characterization - Section 8.4
  • Test Material - Section 9
    • General - Section 9.1
    • Stock Solution - Section 9.2
    • Test Concentrations - Section 9.3
  • Test Organisms - Section 10
    • Species - Section 10.1
    • Age - Section 10.2
    • Source - Section 10.3
    • Brood Stock - Section 10.4
    • Food - Section 10.5
    • Handling - Section 10.6
    • Harvesting Young - Section 10.7
    • Quality - Section 10.8
  • Procedure - Section 11
    • Experimental Design - Section 11.1
    • Dissolved Oxygen - Section 11.2
    • Temperature - Section 11.3
    • Loading - Section 11.4
    • Salinity - Section 11.5
    • Light - Section 11.6
    • Beginning of Test - Section 11.7
    • Feeding - Section 11.8
    • Duration of Test - Section 11.9
    • Biological Data - Section 11.10
    • Other Measurements - Section 11.11
  • Analytical Methodology - Section 12
  • Acceptability of Test - Section 13
  • Interpretation of Results - Section 14
  • Report - Section 15
  • Appendixes
    • Holmesimysis costata - Annex X1
    • Neomysis mercedis - Annex X2

1.6 The values stated in SI units are to be regarded as the standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7.

5.1 Mysids are an important component of both the pelagic and epibenthic community. They are preyed upon by many species of fish, birds, and larger invertebrate species, and they are predators of smaller crustaceans and larval stages of invertebrates. In some cases, they feed upon algae. Mysids are sensitive to both organic and inorganic toxicants (1).3

The ecological importance of mysids, their wide geographical distribution, ability to be cultured in the laboratory, and sensitivity to contaminants make them appropriate acute toxicity test organisms.

5.2 An acute toxicity test is conducted to obtain information concerning the immediate effects of a short-term exposure to a test material on a test organism under specified experimental conditions. An acute toxicity test provides data on the short-term effects that are useful for comparisons to other species but does not provide information on delayed effects.

5.3 Results of acute toxicity tests can be used to predict acute effects likely to occur on aquatic organisms in field conditions except that mysids might avoid exposure when possible.

5.4 Results of acute toxicity tests might be used to compare the acute sensitivities of different species and the acute toxicities of different test materials, and to study the effects of various environmental factors on results of such tests.

5.5 Results of acute toxicity tests might be an important consideration when assessing the hazards of materials to aquatic organisms (see Guide E1023) or when deriving water quality criteria for aquatic organisms (2).

5.6 Results of acute toxicity tests might be useful for studying biological availability of, and structure activity relationships between test materials.

5.7 Results of acute toxicity tests will depend, in part, on the temperature, quality of the food, condition of test organisms, test procedures, and other factors.

SDO ASTM: ASTM International
Document Number E1463
Publication Date Dec. 1, 2012
Language en - English
Page Count 23
Revision Level 92(2012)
Supercedes
Committee E50.47
Loading...

Failed to load document history.

Publish Date Document Id Type View