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Methods for determination of particle size distribution -- Guide to microscope and image analysis methods
This Part of BS 3406 recommends methods for using microscopes to measure the size of particles in the range from 2 nm to 1 mm. Making measurements with light microscopes, scanning electron microscopes and transmission electron microscopes is described both with and without the use of automatic and semi-automatic image analysers.
A system is established for calculating the number of fields of view that have to be analysed to achieve a specified accuracy, and methods are given for the estimation of uncertainties. Guidance is provided on sample preparation, practical microscopy and the choice of equipment for image analysis.
This Part of BS 3406 does not cover the measurement of particle shape or the use of stereorological methods.
This standard does not cover the measurement of asbestos fibres. Asbestos fibres are covered by the European Reference Method (ERM) as given in MDHS 391).
Specialized techniques applicable to the study of cell biology or haematology are outside the scope of this Part of BS 3406.
1) Available from the Health and Safety Executive.
Recommends methods for measuring particles in the range 2 nm to 1 mm using light, scanning electron, and transmission electron microscopes.
Calculation examples are included.
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