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BSI BS EN 12821:2009

Foodstuffs. Determination of vitamin D by high performance liquid chromatography. Measurement of cholecalciferol (D3) or ergocalciferol (D2)
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BSI BS EN 12821:2009

Foodstuffs. Determination of vitamin D by high performance liquid chromatography. Measurement of cholecalciferol (D3) or ergocalciferol (D2)

PUBLISH DATE 2009
PAGES 28
BSI BS EN 12821:2009
This European Standard specifies a method for the determination of vitamin D3 (cholecalciferol) or vitamin D2 (ergocalciferol) in foodstuffs by high performance liquid chromatography (HPLC). Vitamin D3 is primary in foodstuffs of animal origin, while vitamin D2 is primary in wild mushrooms. Both vitamin D3 and vitamin D2 can be present in fortified foodstuffs. This European Standard is not applicable for samples with a content of vitamin D3 and vitamin D2. Apart from the vitamin D activity from the parent forms, vitamin D3 and vitamin D2, the corresponding metabolites 25-hydroxy vitamin D and 1,25-dihydroxy vitamin D also contribute to the vitamin D activity. This European Standard does only include measurement of vitamin D3 or vitamin D2. This European Standard provides the base for the analytical methods. It is intended to serve as a frame in which the analyst can define his own analytical work in accordance to the standard procedure. This method has been validated in inter-laboratory tests on fortified and non-fortified samples such as margarine, milk, milk powder, liquid infant formula, infant formula, cooking oil, and fish oil at levels from 0,4 ?g/100 g to 14 ?g/100 g. Further information on the validation data is given in Annex D.
SDO BSI: British Standards Institution
Document Number EN 12821
Publication Date April 30, 2009
Language en - English
Page Count 28
Revision Level
Supercedes
Committee AW/275
Publish Date Document Id Type View
April 30, 2009 BS EN 12821:2009 Revision
May 15, 2000 BS EN 12821:2000 Revision