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Animal feeding stuffs: Methods of sampling and analysis. Determination of dioxins and dioxin-like PCBs by GC/HRMS and of indicator PCBs by GC/HRMS
This document is applicable to the determination of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), (together termed ādioxinsā (PCDD/Fs)) and dioxin-like PCBs and non-dioxin-like PCBs (dl-PCBs and ndl-PCBs) in animal feeding stuffs. Collaborative studies have been carried out. The method is suitable for the determination of dioxins, dl-PCBs and ndl-PCBs at the appropriate MRL in compound feed and ingredients e.g. oil, mineral clay. The method is applicable to samples containing trace level amounts of one or more dioxins, dioxin-like PCBs and non-dioxin-likePCBs. The limit of quantification (LOQ) is
0,05Ā pg/g (OCDD/ FĀ =Ā 0,1Ā pg/g) for the relevant individual congeners of dioxins/furans,
0,05Ā pg/g for non-ortho PCBs,
10Ā pg/g for mono-ortho PCBs, and
100Ā pg/g for non-dioxin-like-PCBs.
For determination of dioxins and dioxin-like PCBs, the procedure can be used as confirmatory method as defined by Commission Regulation (EC) No 152/2009 for dioxins and dl-PCB in feed [1]. Confirmatory methods as described in this standard are high-resolution gas chromatography/high resolution mass spectrometry (HRGC/HRMS) methods. If only the analysis of non-dioxin-like PCBs is required, a GC-LRMS method can be used (e.g. EN 15741 [2]) provided that appropriate analytical performance criteria are met in the relevant range for the matrix of interest.
This document is split into four modules. Each module describes a part of the whole procedure (see FigureĀ 1 and FigureĀ 2) to be followed:
Module A: Description of standards which might be used;
Module B: Description of extraction procedures;
Module C: Description of clean-up procedures;
Module D: GC/HRMS determination.
Each module describes a part of the whole method as well as, when applicable, alternatives which should be equivalent. Each module has to be regarded as an example. Combining modules and/or alternatives gives a highly flexible, āperformance basedā procedure. It is permitted to modify the method if all performance criteria laid down in CommissionĀ RegulationĀ (EC)Ā NoĀ 152/2009 [1] are met.
Any deviation of the described method, combination of modules needs to be recorded as part of the QA/QC procedures of accredited laboratories and should be available on request.
| SDO | BSI: British Standards Institution |
| Document Number | EN 16215 |
| Publication Date | Jan. 20, 2020 |
| Language | en - English |
| Page Count | 58 |
| Revision Level | |
| Supercedes | |
| Committee | AW/10 |