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BSI BS EN 18033:2024

Food authenticity. Quantitation of equine DNA relative to mammalian DNA in raw beef (meat)
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BSI BS EN 18033:2024

Food authenticity. Quantitation of equine DNA relative to mammalian DNA in raw beef (meat)

PUBLISH DATE 2025
PAGES 16
BSI BS EN 18033:2024
This document specifies a real-time PCR procedure for the quantitation of the amount of equine DNA relative to total mammalian DNA in a raw meat sample. Results of this equine assay are expressed in terms of equine (Equus genus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. This assay is specific for representatives of the genus Equus and therefore detects horse, mule, donkey and zebra DNA. The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw horse meat in a raw beef (meat) background. The limit of detection has been determined experimentally to be at least 17 horse haploid genome equivalents (HGE) for both the equine PCR and the mammalian PCR based on the lowest dilution on the respective calibration curves through single laboratory validation. The lowest relative horse content of the target sequence included in the collaborative study was a mass fraction of 0,1 % based on gravimetrically prepared raw horse muscle tissue in a raw beef muscle tissue background. The compliance assessment process is not part of this document.
SDO BSI: British Standards Institution
Document Number EN 18033
Publication Date Jan. 13, 2025
Language en - English
Page Count 16
Revision Level
Supercedes
Committee AW/34/46
Publish Date Document Id Type View
Jan. 13, 2025 BS EN 18033:2024 Revision