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BSI BS EN 18057:2025

Food authenticity. Quantitation of roe deer DNA relative to mammalian DNA in meat and meat products by real-time PCR
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BSI BS EN 18057:2025

Food authenticity. Quantitation of roe deer DNA relative to mammalian DNA in meat and meat products by real-time PCR

PUBLISH DATE 2025
PAGES 20
BSI BS EN 18057:2025
This document specifies a real-time PCR procedure for the quantitation of the amount of roe deer DNA relative to total mammalian DNA in meat and meat products. Results of this roe deer assay are expressed in terms of roe deer (Capreolus capreolus) haploid genome copy numbers relative to total mammalian haploid genome copy numbers. The content of roe deer can also be expressed as mass fraction in % using gravimetrically prepared calibration material from meat mixtures or model samples. The method has been previously validated in a collaborative study and applied to DNA extracted from samples that consist of raw roe deer meat in a raw pig meat background as well as raw and boiled sausages. The limit of detection of the roe deer PCR has been determined experimentally to be at least 5 target gene copies or at least 0,1 % roe deer. The compliance assessment process is not part of this document.
SDO BSI: British Standards Institution
Document Number EN 18057
Publication Date June 25, 2025
Language en - English
Page Count 20
Revision Level
Supercedes
Committee AW/34/46
Publish Date Document Id Type View
June 25, 2025 BS EN 18057:2025 Revision