BSI BS EN ISO 23418:2022

Current Revision
Microbiology of the food chain. Whole genome sequencing for typing and genomic characterization of bacteria. General requirements and guidance

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BSI BS EN ISO 23418:2022

Microbiology of the food chain. Whole genome sequencing for typing and genomic characterization of bacteria. General requirements and guidance

PUBLISH DATE 2022
PAGES 58
BSI BS EN ISO 23418:2022

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain.

This process can include the following stages:

  • a) handling of bacterial cultures;
  • b) axenic genomic DNA isolation;
  • c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage;
  • d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation;
  • e) metadata capture and sequence repository deposition;
  • f) validation of the end-to-end WGS workflow (fit for purpose for intended application).

This document is applicable to bacteria isolated from:

  • products intended for human consumption;
  • products intended for animal feed;
  • environmental samples from food and feed handling and production areas;
  • samples from the primary production stage.
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