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Microbiology of food and animal feeding stuffs. Horizontal method for the detection and enumeration of coliforms. Most probable number technique
This International Standard gives general guidelines for the detection and the enumeration of coliforms. It is applicable to:
products intended for human consumption and for the feeding of animals, and
environmental samples in the area of food production and food handling.
Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.
NOTE The temperature is subject to agreement between the parties concerned. In the case of milk and milk products, the temperature of incubation is 30 °C.
This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.
A limitation on the applicability of this International Standard is imposed by the susceptibility of the method to a large degree of variability. The information given in Clause 11 provides guidance on the applicability of the method and on the interpretation of the results.
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