BSI BS EN ISO 9167-1:1995+A1:2013

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Rapeseed. Determination of glucosinolates content -- Method using high-performance liquid chromatography

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BSI BS EN ISO 9167-1:1995+A1:2013

Rapeseed. Determination of glucosinolates content -- Method using high-performance liquid chromatography

PUBLISH DATE 2013
PAGES 14
BSI BS EN ISO 9167-1:1995+A1:2013

This part of ISO 9167 specifies a method for the determination of the content of the different glucosinolates in rapeseeds (colza) using high-performance liquid chromatography.

NOTE 1: This method does not determine glucosinolates which are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed.

NOTE 2: A rapid method for the determination of glucosinolates content using X-ray fluorescence spectrometry is the subject of ISO 9167-2.

Purification and enzymatic desulfatation of a methanol extract on ion exchange resin followed by reverse phase HPLC technique with UV detection.

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