This Document Has Been Superseded
A newer publication replaces it: BSI BS EN ISO 9167
Rapeseed. Determination of glucosinolates content -- Method using high-performance liquid chromatography
This part of ISO 9167 specifies a method for the determination of the content of the different glucosinolates in rapeseeds (colza) using high-performance liquid chromatography.
NOTE 1: This method does not determine glucosinolates which are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed.
NOTE 2: A rapid method for the determination of glucosinolates content using X-ray fluorescence spectrometry is the subject of ISO 9167-2.
Purification and enzymatic desulfatation of a methanol extract on ion exchange resin followed by reverse phase HPLC technique with UV detection.
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