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Animal feeding stuffs. Methods of sampling and analysis. PFGE typing of Lactobacilli, Pediococci, Enterococci and Bacilli in animal feeds
This document defines a Pulsed Field Gel Electrophoresis (PFGE) methodology for the identification of authorized probiotic Lactobacillus, Pediococcus, Enterococcus and Bacillus strains.
The method can be applied to purified colonies obtained from cultured premixtures and feeds, in order to verify the presence of strains used as feed additives in declared concentrations, even against eventual microbial background resulting from nonsterile matrices.
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