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Nanotechnologies. Guidelines for sample preparation, detection, identification and characterization by spICP-MS and EM-EDX of nano-objects in inorganic additives incorporated in food matrices
This document provides guidance to the food industry, service providers and control laboratories on methodologies to be used for sample preparation, detection, identification and measurement of nano objects in inorganic food additives incorporated in food matrices.
Electron microscopy combined with energy dispersive X-ray spectroscopy (EM-EDX) and inductively coupled plasma mass spectrometry (ICP-MS) operated in single particle mode (spICP-MS) are the selected measurement methodologies to provide information on:
Special attention is given to the sample preparation, including matrix digestion, sample extraction and dilution steps to be used according to the combination of:
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