BSI PD CEN ISO/TS 21569-10:2026

Current Revision
Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products -- Construct- and event-specific detection methods for genetically modified salmon expressing CS-GHc2 growth hormone

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BSI PD CEN ISO/TS 21569-10:2026

Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products -- Construct- and event-specific detection methods for genetically modified salmon expressing CS-GHc2 growth hormone

PUBLISH DATE 2026
PAGES 20
BSI PD CEN ISO/TS 21569-10:2026

This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture.

The genetically modified AquAdvantage Atlantic salmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either:

  • the border between the growth hormone coding sequence (CS-GHc2) of Oncorhynchus tshawytscha (Chinook salmon) and the antifreeze terminator (T-AFP) of (Macro-) Zoarces americanus (ocean pout), i.e. with the construct-specific method, or
  • the border between the Atlantic salmon genomic DNA and the antifreeze promoter (P-AFP) of ocean pout, i.e. with the event-specific method.

These methods can be applied to identify the genetically modified (GM) fish or for screening purposes.

This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs.

The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.

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