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Horizontal methods for molecular biomarker analysis. Methods of analysis for the detection of genetically modified organisms and derived products -- Construct- and event-specific detection methods for genetically modified salmon expressing CS-GHc2 growth hormone
This document specifies procedures for the detection of a DNA sequence of a construct used to (genetically) enhance the growth of fish commonly found in aquaculture.
The genetically modified AquAdvantage Atlantic salmon (Salmo salar) carries the construct expressing CS-GHc2 growth hormone and can be detected based on a real-time polymerase chain reaction (PCR) targeting either:
These methods can be applied to identify the genetically modified (GM) fish or for screening purposes.
This document is applicable for the analysis of DNA extracted from foodstuffs. It can also be suitable for the analysis of DNA extracted from other products such as feedstuffs.
The application of these methods requires the extraction of an adequate amount of amplifiable DNA from the relevant matrix.
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