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BSI PD ISO/TS 20224-2:2020

Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR -- Ovine DNA detection method
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BSI PD ISO/TS 20224-2:2020

Molecular biomarker analysis. Detection of animal-derived materials in foodstuffs and feedstuffs by real-time PCR -- Ovine DNA detection method

PUBLISH DATE 2020
PAGES 26
BSI PD ISO/TS 20224-2:2020

This document specifies a real-time polymerase chain reaction (real-time PCR) method for the qualitative detection of ovine-specific DNA derived from food and feed. It requires the extraction of an adequate amount of PCR amplifiable DNA from the relevant matrix and can be applied to the detection of ovine material derived from sheep (Ovis aries).

The target sequence is a partial fragment of the ovine nuclear prolactin receptor short form mRNA gene (PRLR) (i.e. GenBank accession number AF041979.1)[ 1], which is present as a single copy per haploid genome. The provided PCR assay for this target has an absolute limit of detection of five copies per reaction, with ? 95 % replicability at this concentration (LOD95 %).

SDO BSI: British Standards Institution
Document Number ISO/TS 20224-2
Publication Date Aug. 13, 2020
Language en - English
Page Count 26
Revision Level
Supercedes
Committee AW/275
Publish Date Document Id Type View
Aug. 13, 2020 PD ISO/TS 20224-2:2020 Revision