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Milk and milk products - Enumeration of presumptive Escherichia coli - Part 1: Most probable number technique using 4-methylumbelliferyl-beta-D-glucuronide (MUG)
ISO 11866-1|IDF 170-1:2005 specifies a combined method for the enumeration of presumptive Escherichia coli and of presumptive coliforms by means of a culture technique involving a liquid medium with MUG, and calculation of the number of presumptive Escherichia coli and/or coliforms per gram or per millilitre by the most probable number (MPN) technique after incubation at 30 °C.
The method is applicable to:
The method specified in ISO 11866-1|IDF 170-1:2005 is preferred for samples in which comparatively low numbers of presumptive Escherichia coli and/or other presumptive coliforms (less than 100 per gram or 10 per millilitre) are suspected.
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