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ISO 21446:2019

Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC
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ISO 21446:2019

Infant formula and adult nutritionals - Determination of trans and total (cis + trans) vitamin K1 content - Normal phase HPLC

PUBLISH DATE 2019
ISO 21446:2019

This document specifies a method for the quantitative determination of trans and total (cis + trans) vitamin K1 in infant, pediatric and adult nutritionals using normal phase (NP) high-performance liquid chromatography (HPLC) with post-column reduction and fluorescence detection.

The method demonstrated good linearity over a standard range of ~2 ÎĽg/l to 80 ÎĽg/l trans vitamin K1, and the limit of quantification (LOQ) was estimated to be 0,4 ÎĽg/l for standards and 0,09 ÎĽg/100 g ready to feed (RTF) for samples assuming 4 grams of sample are diluted to 10 ml.

SDO ISO: International Organization for Standardization
Document Number ISO 21446
Publication Date Jan. 1, 2019
Language en - English
Page Count
Revision Level
Supercedes
Committee ISO/TC 34
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